Composite

Part:BBa_J15510:Design

Designed by: Kwabena O. Duedu, Chris French, Sahreena Lakhundi   Group: French Lab   (2013-11-25)


Pspac+RBS+cex+RBS+cenA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 818
    Illegal NotI site found at 1799
    Illegal NotI site found at 2943
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2856
    Illegal BamHI site found at 1992
    Illegal XhoI site found at 2354
    Illegal XhoI site found at 2603
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 451
    Illegal NgoMIV site found at 824
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 2134
    Illegal NgoMIV site found at 3059
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2038
    Illegal BsaI.rc site found at 871
    Illegal SapI.rc site found at 954


Design Notes

Cex is a GC rich part and requires extended denaturation with glycerol or commercial GC enhancers


Source

Cellulomonas fimi

References